Investigation of the preventative roles of manganese superoxide dismutase Ala16Val gene polymorphism in coronary artery events

Hale Atmaca1, Meliha Koldemir Gündüz1, Penbe Çağatay2, Mehtap Çevik1, Belgin Süsleyici Duman1

1Marmara Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Moleküler Biyoloji Anabilim Dalı, İstanbul, Türkiye
2İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Biyoistatistik ve Tıp Bilişimi Anabilim Dalı, İstanbul, Türkiye

Keywords: Ala16Val polymorphism; coronary artery disease; MnSOD gene.

Abstract

Objectives: The aim of this study was to determine the genotypic frequencies of manganese superoxide dismutase (MnSOD) gene Ala16Val polymorphism frequencies and to evaluate the effects of MnSOD Ala16Val variation over coronary artery disease (CAD) in which people, biochemical parameters and MnSOD activity.
Materials and methods: MnSOD gene Ala16Val polymorphisms were determined with quantitative polymerase chain reaction method. MnSOD activity in serum were determined by ELISA method.
Results: The MnSOD gene Ala16Val genotype frequencies were determined respectively as 20.9%, 41.9%, 37.2% for homozygous wild genotype (C/C), heterozygous genotypes (C/T), homozygous polymorphic genotype (T/T) in the CAD patients; whereas 15.5%, 51.7% and 32.8% for the patients without CAD. In total study group, C/C genotype carriers were detected to have the highest, whereas T/T genotype carriers were detected to have the lowest mitochondrial MnSOD activities when compared according to MnSOD gene Ala16Val genotypes. In the patients without CAD, MnSOD Ala16Val polymorphism was found to have significant effects on lean body mass (p=0.01) and fat mass (p=0.05). In CAD patients with Ala16Val polymorphism heterozygous genotype oil-rich eating habits were lower to that of other genotypes, whereas CAD patients with homozygous polymorphic genotype was detected to prefer protein-poor diet.
Conclusion: When all the study group was evaluated, MnSOD Ala16Val genotypes were not found to have statistically significant relationship with eating habits, but found to be effective only on lean body mass.