Immunohistochemical restaining of hematoxylin and eosin stained slides: a histotechnical study
Türker Çavuşoğlu1,2, Kubilay Doğan Kılıç1, Aylin Buhur1, Yasemin Adalı1, Gürkan Yiğittürk1, Oytun Erbaş3, Yiğit Uyanikgil1,2
1Ege Üniversitesi Tıp Fakültesi Histoloji ve Embriyoloji Anabilim Dalı, İzmir, Türkiye
2Ege Üniversitesi Kordon Kanı, Hücre-Doku Uygulama ve Araştırma Merkezi, İzmir, Türkiye
3İstanbul Bilim Üniversitesi Tıp Fakültesi, Fizyoloji Anabilim Dalı, İstanbul, Türkiye
Keywords: Hematoxylin and eosin staining; histochemical staining; immunohistochemical staining; restraining.
Abstract
Objectives: This study aims to set up a protocol that makes the removal of the stain possible and that it is suitable for different histochemical and immunohistochemical stains in a hematoxylin and eosin stained slide.
Materials and methods: Hematoxylin and eosin staining was removed with the developed protocol, followed by anti-iNOS staining, in this technical study done by using rat liver and kidney slides.
Results: We detected no residual of histological stains on the hematoxylin and eosin stained slides when the developed protocol was applied. The basic structures of rat liver and kidney tissues were displayed in the slides stained with hematoxylin and eosin without any exposure to degradation caused by the protocol process.
Conclusion: In this study, we showed that it was possible to economically and effectively restain the unstained slides with the developed protocol, both histochemically and immunohistochemically, and to effectively re-evaluate the slides in the archives of histology laboratories.