Genotyping and analysis of rs3771150 polymorphism to investigate an association with bronchopulmonary dysplasia incidence in Turkish population
Ayberk Akat1, Seda Yılmaz Semerci2, Osman Mutluhan Uğurel3, Ayşegül Erdemir4, Merih Çetinkaya2, Dilek Turgut-Balık1
1Department Bioengineering, Yıldız Technical University, Chemistry and Metallurgy Faculty, İstanbul, Turkey
2Department of Neonatal Intensive Care Unit, Health Sciences University, İstanbul Kanuni Sultan Süleyman Training and Research Hospital, İstanbul, Turkey
3Department of Basic Sciences, Altınbaş University, School of Engineering and Natural Sciences, İstanbul, Turkey 4Department Molecular Biology, Yıldız Technical University, Faculty of Arts and Science, İstanbul, Turkey
Keywords: Bronchopulmonary dysplasia; multiplex polymerase chain reaction; single nucleotide polymorphism; SNP genotyping.
Objectives: rs3771150 polymorphism on IL-18RAP was chosen to be investigated in terms of determining a relationship with BPD incidence seen in preterm infants born in Turkey.
Materials and Method: Allele and genotype frequencies of this polymorphism was analyzed from genotyping results obtained by multiplex reactions and MALDI-TOF mass spectrometry method. In addition, clinical and demographic data obtained from the patients were also inquired in this study to determine any relationship with BPD incidence.
Results: GG genotype has been determined as the wild type genotype of rs3771150 polymorphism for the Turkish population (n=49 in control group, n=46 in BPD group). When other genotypes (AG and AA) were analyzed no significant relationship was found with BPD incidence (p>0.05).
Conclusion: No significant relationship could be determined between rs3771150 polymorphism and BPD incidence in 192 preterm Turkish infants.