Gülten Karabekir1, Günnur Demircan2, Şule Özdaş2

1İstanbul Bilim Üniversitesi Sağlık Bilimleri Enstitüsü Tıbbi Biyoloji ve Genetik Anabilim Dalı, İstanbul, Türkiye
2İstanbul Bilim Üniversitesi Tıp Fakültesi Tıbbi Biyoloji ve Genetik Anabilim Dalı, İstanbul, Türkiye

Keywords: Annexin V; apoptosis; MCF-7; resveratrol.


Objectives: This study aims to examine the cytotoxic effect of resveratrol, as a natural polyphenolic compound in vitro environment, in MCF-7 breast cancer cell strain.

Materials and methods: We determined the doses of resveratrol with 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT) analysis. We detected the apoptotic effect of resveratrol on MCF-7 cell strain by the imaging flow cytometry using the APC annexin V antibody.

Results: 100 μM and 250 μM resveratrol were incubated in MCF-7 cells and measurements were obtained at the 12, 24 and 48 hours. From the MTT results, we found that the cell density decreased with respect to the control group, directly proportional to the increasing concentration of resveratrol. We determined the cytotoxic doses to be between 250-500 μM in the analysis of the 12th hour, between 100-250 μM at the 24 hour measurement and 100 μM at the 48 hour measurement. When the results of Annexin V flow cytometry measurements were evaluated; we observed that 100 and 250 μM resveratrol given to MCF-7 cells increased early apoptosis compared to the control group.

Conclusion: We marked that the apoptotic effect of resveratrol, as one of all its anticancer effects, especially induced early apoptosis in the MCF-7 breast cancer cell strain depending on both dose and time.