Disodium Pentaborate Decahydrate up-regulates expressions of MAP kinase genes in human prostate cancer cells
Çığır Biray Avcı1, Burcu Erbaykent Tepedelen2, Özgün Özalp1, Bakiye Göker Bağca1, Yavuz Dodurga3, Duygu Aygüneş1, Nur Selvi Günel1, Mehmet Korkmaz4, Cumhur Gündüz1
1Department of Medical Biology, Medical Faculty of Ege University, İzmir, Turkey
2Department of Molecular Biology and Genetic, Avrasya University, Faculty of Science and Letter, Trabzon, Turkey
3Department of Medical Biology, Medical Faculty of Pamukkale University, Denizli, Turkey
4Department of Medical Biology, Medical Faculty of Celal Bayar University, Manisa, Turkey
Keywords: Disodium pentaborate decahydrate; gene expression; prostate cancer.
Objectives: This study aims to investigate the expressions of MAP2K3, MAP3K7, and MAPK8 genes after disodium pentaborate decahydrate (DPD) treatment in DU-145 cells.
Materials and methods: Effect of DPD treatment on expressions of MAP2K3, MAP3K7, and MAPK8 genes were determined by qRT-PCR.
Results: We determined that disodium pentaborate decahydrate treatment increased the expressions of MAP2K3, MAP3K7, and MAPK8 genes in terms of mRNA levels.
Conclusion: The reason of increased apoptosis might be associated with high expression levels of MAP2K3, MAP3K7, and MAPK8 genes after DPD treatment. Our novel findings suggest that DPD may be an important agent in the treatment of prostate cancer by inducing apoptosis against mitogenic and environmental stress.