Disodium Pentaborate Decahydrate up-regulates expressions of MAP kinase genes in human prostate cancer cells

Çığır Biray Avcı; Burcu Erbaykent Tepedelen; Özgün Özalp; Bakiye Göker Bağca; Yavuz Dodurga; Duygu Aygüneş; Nur Selvi Günel; Mehmet Korkmaz; Cumhur Gündüz

doi:10.5606/fng.btd.2016.019

Çığır Biray Avcı¹, Burcu Erbaykent Tepedelen², Özgün Özalp¹, Bakiye Göker Bağca¹, Yavuz Dodurga³, Duygu Aygüneş¹, Nur Selvi Günel¹, Mehmet Korkmaz⁴, Cumhur Gündüz¹

¹Department of Medical Biology, Medical Faculty of Ege University, İzmir, Turkey
²Department of Molecular Biology and Genetic, Avrasya University, Faculty of Science and Letter, Trabzon, Turkey
³Department of Medical Biology, Medical Faculty of Pamukkale University, Denizli, Turkey
⁴Department of Medical Biology, Medical Faculty of Celal Bayar University, Manisa, Turkey

Keywords: Disodium pentaborate decahydrate; gene expression; prostate cancer.

Abstract

Objectives: This study aims to investigate the expressions of MAP2K3, MAP3K7, and MAPK8 genes after disodium pentaborate decahydrate (DPD) treatment in DU-145 cells.
Materials and methods: Effect of DPD treatment on expressions of MAP2K3, MAP3K7, and MAPK8 genes were determined by qRT-PCR.
Results: We determined that disodium pentaborate decahydrate treatment increased the expressions of MAP2K3, MAP3K7, and MAPK8 genes in terms of mRNA levels.
Conclusion: The reason of increased apoptosis might be associated with high expression levels of MAP2K3, MAP3K7, and MAPK8 genes after DPD treatment. Our novel findings suggest that DPD may be an important agent in the treatment of prostate cancer by inducing apoptosis against mitogenic and environmental stress.